Figure 3 | Scientific Reports

Figure 3

From: Therapy of corneal endothelial dysfunction with corneal endothelial cell-like cells derived from skin-derived precursors

Figure 3

Characteristic of subcultured CEC-like cells. (A) Subcultured CEC-like cells formed a monolayer of hexagonal and pentagonal cells through 10 days of culture. (B–E) Immunofluorescent staining, Western blotting, and RT-PCR showed that the passaged CEC-like cells continued to express CECs markers. All samples derived from the same experiment were processed in parallel. One representative experiment out of three is presented. Cropped images were displayed and full-length blots are shown in the Supplementary Figure 5. Scale bars 100 μm. Results are mean ± S.E.M. *p < 0.05 compared to SKPs.

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