Figure 4
From: Herpud1 negatively regulates pathological cardiac hypertrophy by inducing IP3 receptor degradation
Herpud1 siRNA triggers cardiomyocyte hypertrophy. (a) Representative Western blot of Herpud1 protein levels in isolated neonatal (Neo) and adult (Adu) cardiomyocytes (Cardio) and neonatal fibroblasts (Fibro) cultured under basal conditions or treated with tunicamycin for 4 h (Tn, 1.2 mM). Ponceau red shows the loading of each sample (n = 3). (b) Representative images (400X) of fixed NRVM stained with the fluorescent probe rhodamine-phalloidin after incubation with unrelated siRNA (UNR, 200 nM) or anti-Herpud1 siRNA (siHerpud1 #1) (200 nM) for 48 h (n = 3). (c) White arrows show analyzed sarcomeres in the fluorescent profiles of sarcomeres from the previous images. (d) Results for area, perimeter, and percentage of sarcomerized cells for fixed NRVM stained with rhodamine-phalloidin after incubation with unrelated siRNA (UNR) or anti-Herpud1 siRNA (siHerpud1 #1) for 48 h (n = 3). (e) Representative Western blots for Herpud1 (n = 6), β-MHC (n = 6), and Rcan1.4 (n = 4) for NRVM incubated with unrelated siRNA (UNR) or anti- Herpud1 siRNA (siHerpud1#2) for 48 h. Graphs represent the measurements for each protein using β-tubulin as the loading control. Mean ± SEM, analyzed using a t-test. *p < 0.05 vs. UNR.
