Figure 5
From: Herpud1 negatively regulates pathological cardiac hypertrophy by inducing IP3 receptor degradation
IP3R protein and mRNA levels in cardiomyocytes treated with Herpud1 siRNA and in hearts from Herpud1-KO mice. (a) Representative Western blot of IP3R protein levels in Left: control (UNR) and Herpud1-knockdown NRVM (siHerpud1 #2, 48 h) (n = 6) and Right: samples from wild-type (WT) and Herpud1-knockout (Herpud1 KO) mice (n = 3). (b) Graphs show the relative expression of IP3R1, IP3R2, and IP3R3 mRNA in control (UNR) and Herpud1-knockdown NRVM (siHerpud1 #2, for 48 h) (n = 4) and IP3R2 mRNA in cardiac samples from wild-type (WT) and Herpud1-knockout (Herpud1 KO) 10-to-12-week-old mice (n = 3). Pabpn1 was used as a housekeeping messenger in isolated NRVM, and the 18 S ribosomal subunit was used in the cardiac tissue samples. Mean ± SEM, analyzed using a t-test. *p < 0.05 vs. UNR.
