Figure 4
From: TIPE2 negatively regulates mycoplasma pneumonia-triggered immune response via MAPK signaling pathway
TIPE2 negatively regulated MP-triggered inflammatory cytokines production in macrophages. After stimulating with 100 nM PMA, THP-1 cells were transfected with control siRNA (NC) or TIPE2 siRNA, as indicated at a final concentration of 20 nM. 48 h later, cells were stimulated with or without MP (50 CFU/cell). IL-6 (a), TNF-α (c), IL-1β (e) mRNA levels were measured at 4 h and 8 h by qPCR after stimulation. Supernatants were collected after 12 h and 24 h to measure IL-6 (b), TNF-α (d), IL-1β (f) by ELISA. Data are the mean ± S.D. (n = 3) of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.
