Figure 6
From: TIPE2 negatively regulates mycoplasma pneumonia-triggered immune response via MAPK signaling pathway
TIPE2 inhibits MP-triggered inflammatory cytokines production via inhibiting MAPK activation. After stimulating with 100 nM PMA, THP-1 cells were transfected with control siRNA (NC) or TIPE2 siRNA, as indicated at a final concentration of 20 nM. The transfectants of THP-1 cells were preincubated with 30 μM SP600125, SB203580 or PD98059 for 3 h before being stimulated with 50 CFU/cell MP. IL-6 (a), TNF-α (c), IL-1β (e) mRNA levels were measured at 8 h by qPCR after stimulation. Supernatants were collected after 12 h to measure IL-6 (b), TNF-α (d), IL-1β (f) by ELISA. Data are the mean ± S.D. (n = 3) of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.
