Figure 5

The representative images of attachment, viability and distribution of BMMSCs in composite scaffolds (A). Blue fluorescence represents the contours of scaffolds, merged images included bright filed views to show the pores of the scaffolds. Confocal microscopic images of Live/Dead staining demonstrated cell viability of three groups after 72 hours of culture. The distribution of MSCs on the PCL strands and on the pores and strands of hybrid scaffolds were clearly displayed (Red, dead cells; green, live cells; Scale bar = 250 μm). CCK-8 assay showed that the number of cells in the three groups increased over time (B). DNA content in the various scaffolds during osteogenic culture indicating slow proliferation while MSCs differentiating into osteoblasts. Results are expressed as mean ± SD, (n = 3, *^,#P < 0.05, **^,##P < 0.01; ^#compared to PCL group in (B), and compared to day 1 in (C)).