Table 2 Data collection and refinement statistics for the fucosylated IgG1-Fc/sFcγRIIIa complexes.
Fucosylated IgG1-Fc/sFcγRIIIa | Fucosylated IgG1-Fc- Y296W/sFcγRIIIa | |
|---|---|---|
Crystallographic data | ||
Space group | P41212 | P41212 |
Unit cell a/b/c (Å) | 77.6/77.6/351.9 | 77.3/77.3/349.8 |
Data processing statistics | ||
Beam line | SPring-8 BL44XU | PF BL5A |
Wavelength (Å) | 0.90000 | 1.00000 |
Resolution (Å) | 46.78–2.40 (2.54–2.40) | 46.55–2.50 (2.65–2.50) |
Total/unique reflections | 487,882/43,217 | 512,558/37,895 |
Completeness (%) | 99.5 (97.1) | 99.7 (98.9) |
R merge (%) | 10.4 (61.1) | 7.7 (90.2) |
I/σ (I) | 14.2 (1.6) | 23.7 (2.6) |
CC 1/2 | 0.998 (0.777) | 0.999 (0.801) |
Refinement statistics | ||
Resolution (Å) | 20.0–2.40 | 20.0–2.50 |
R work/R free (%) | 23.3/28.1 | 22.0/27.0 |
Number of non-Hydrogen atoms | ||
Protein [Fc(A)/Fc(B)/FcR(C)] | 1700/1712/1225 | 1714/1714/1225 |
Water | 109 | 109 |
Sugar [Fc(A)/Fc(B)/FcR(C)] | 110/110/63 | 110/110/78 |
R.m.s. deviations from ideal | ||
Bond lengths (Å) | 0.010 | 0.011 |
Bond angles (°) | 1.57 | 1.61 |
Ramachandran plot (%) | ||
Most favored regions | 93.4 | 91.2 |
Additionally allowed regions | 6.2 | 8.6 |
Generously allowed regions | 0.4 | 0.2 |
Disallowed | 0 | 0 |
Average B-factors (Å2) | ||
Protein [Fc(A)/Fc(B)/FcR(C)] | 56.6/44.3/70.9 | 66.8/57.2/81.4 |
Water | 47.3 | 54.5 |
Sugar [Fc(A)/Fc(B)/FcR(C)] | 67.1/63.5/106.2 | 77.1/77.1/127.2 |
