Figure 3

Translation efficiency is a determinant of the magnitude of miRNA-mediated translational repression but not RNA degradation of endogenous miRNA targets. Cumulative distributions of fold change of RPF (ribosome protected fragments), (A,C) or fold change of RNA, (B) for all miR-155 predicted targets (http://targetscan.org/) in data from Guo et al., 2010. Fold change is calculated as the log2 normalized RPF or RNAseq reads for miR-155 transfected divided by mock transfected. TE for each transcript in the absence of miR-155 (mock transfection) was calculated by normalized RPF divided by normalized RNAseq reads. All miR-155 targets are binned by TE, above or below the median (“High” or “Low”), A and B or by TE quartiles, (C)(D,F) Correspondence between RPF fold change and TE, D, or RNA fold change and TE, F. E miR-155 targets were binned by the number of conserved and poorly conserved binding sites for miR-155 as well as TE. **p < 0.01, ***p < 0.001 by Kolmogorov–Smirnov test.