Figure 2 | Scientific Reports

Figure 2

From: Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis

Figure 2

Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve (a) and the melt peak (b). For the Bst DNA polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6 U and 8 U, respectively. Ratios of inner to outer primers were set at 2:1, 4:1, 6:1 and 8:1 with the outer primer concentration fixed to 0.2 μM to optimize the primer ratios. Mg2+ concentrations in the LAMP reactions were varied from 5 mM, to 8 mM for the optimization of Mg2+.

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