Figure 5
Sensitivity comparison of Ustilago maydis RealAmp and real-time PCR using the SG200 strain plasmid DNA as template. (a) Sensitivity of the RealAmp assay conducted on the Bio-Rad CFX96 Real-Time PCR system. The RealAmp fluorescence (RFU) vs. Cq [threshold time (min)] amplification curve graph and standard curve in which threshold time was plotted against the logarithmic amount of initial template DNA were plotted automatically by the CFX96 Real-Time PCR system. (b) Sensitivity test of real-time PCR for detecting U. maydis. The real-time fluorescence units were plotted against the initial concentration of plasmid DNA ranging from 4.4 × 10−1 ng/μl to 4.4 × 10−7 ng/μl by the CFX96 Real-Time PCR system. The standard curve was generated using known Log10-transformed concentrations of a 10-fold serial dilution of the pEasy-Pep plasmid DNA and the corresponding threshold cycle (Ct) values. (c) RealAmp sensitivity test using ZYD-S1 instrument. The fluorescence vs. time amplification curves of RealAmp were plotted automatically by the ZYD-S1 Tube Scanner. The standard curve was calculated by ZYD-S1 in which threshold time (Tt) was plotted against the logarithmic amount of initial template plasmid DNA.
