Figure 4 | Scientific Reports

Figure 4

From: Reconstruction of Toll-like receptor 9-mediated responses in HEK-Blue hTLR9 cells by transfection of human macrophage scavenger receptor 1 gene

Figure 4

Secreted embryonic alkaline phosphatase (SEAP) release from untreated, mock-transfected, or MSR1-transfected HEK-Blue hTLR9 cells using HEK-Blue detection solution. Each DNA sample was added to the cells at a final concentration of 50 μg/mL, and the OD of the sample was measured at 620 nm. All oligodeoxynucleotides (ODNs) have a phosphodiester backbone except for CpG2006. The results are expressed as means + SEM of three independent experiments. *P < 0.05 compared with the mock-transfected group.

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