Figure 3
BVDV antigen-specific antibody responses following vaccination with nanoparticulate formulation of BVDV antigens and challenge infection. Sera from calves vaccinated with either inactivated BVDV vaccine (IAV) (squares), Vaccine-NP (triangles) or Control-NP (circles) were collected longitudinally over the course of vaccination and BVDV challenge. NS3 (A) and E2 (B) specific antibodies were measured by competitive ELISA and BVDV-neutralising titres determined by virus-neutralization assay (C). Mean data for each group (n = 6) are presented, error bars represent SEM and statistical analysis was performed using a two-way ANOVA followed by a Tukey’s multiple comparison test; statistically significant differences between the Control-NP group and the IAV and Vaccine-NP groups are indicated as ‘a’ and ‘b’, respectively. Booster immunisation and BVDV challenge infections on days 21 and 41 post-vaccination are indicated.
