Figure 4
BVDV antigen-specific T cell IFN-γ responses following vaccination with nanoparticulate formulation of BVDV antigens and challenge infection. PBMCs from calves vaccinated with either inactivated BVDV vaccine (IAV) (squares), Vaccine-NP (triangles) or Control-NP (circles) were collected longitudinally over the course of vaccination and BVDV challenge, stimulated with recombinant BVDV E2 (A) or NS3 helicase (B) proteins. After 48 hours, IFN-γ in culture supernatants was measured by ELISA. Mean unstimulated-corrected data for each group (n = 6) are presented, error bars represent SEM. Booster immunisation and BVDV challenge infections on days 21 and 41 post-vaccination are indicated.
