Figure 4
Involvement of Dab2 in VEGF receptor endocytosis, regulating LSEC dedifferentiation, proliferation and migration (A) Representative SEM images of fenestrae in SK-HEP1 from NaAsO2- VEGF- siDab2-, NaAsO2 + VEGF- siDab2-, NaAsO2 + VEGF + siDab2- and NaAsO2 + VEGF + siDab2 + group in vitro. Bar = 2μm. All experiments were repeated at least three times. (B) Representative EdU staining images in SK-HEP1 groups described above in vitro. Bar = 50μm. All experiments were repeated at least three times. (C) Representative crystal violet staining images of migrated cells in SK-HEP1 groups described above. Bar = 100μm. All experiments were repeated at least three times. (D) Quantitative porosity of fenestrae in SK-HEP1 groups described above in vitro. **P < 0.01. (E) Cell numbers per field of EdU stained SK-HEP1 groups described above in vitro. **P < 0.01. (F) Cell numbers per field of crystal violet stained SK-HEP1 groups described above in vitro. **P < 0.01.
