Figure 5 | Scientific Reports

Figure 5

From: Cellular hormetic response to 27-hydroxycholesterol promotes neuroprotection through AICD induction of MAST4 abundance and kinase activity

Figure 5

APP ablation and palmitate-rich diet decreased AICD/MAST4/FOXO1/RTKN2 signaling in vivo. AICD binding to MAST4 promoter (A), MAST4 mRNA abundance (B), FOXO1 binding to the RTKN2 promoter (C), RTKN2 mRNA abundance (D), and immunoblotting of MAST4 and RTKN2 (upper panel) with quantification (lower panel) (E), in APP+/+ and APP−/− mouse cortical samples. (A–E) N = 3 independent experiments. AICD binding to the MAST4 promoter (F), MAST4 mRNA abundance (G), FOXO1 binding to the RTKN2 promoter (H), RTKN2 mRNA abundance (I), and immunoblotting of MAST4 and RTKN2 (J) and quantification (K), from the cortices of mice fed standard or palmitate diet. ChIP and mRNA abundance are represented as fold change measurements (FC). (F–K) N = 5 independent experiments. *P < 0.05 significance is in comparison to the APP−/− genetic background (A), APP+/+ genetic background (B–E), or standard diet (F–I and K).

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