Table 1 Sequences and Final Concentrations of Primers and Probes. Access Numbers in Between Parentheses.
From: Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes
Templates | Primers and probes | Final Concentration |
|---|---|---|
Per1 (NM_0011065.3) | Forward: 5′-AGCAGGTTCAGGCTAACCAGGAAT-3′ | 300 nM |
Reverse: 5′-AGGTGTCCTGGTTTCGAAGTGTGT-3′ | 300 nM | |
Probe:5′-/6FAM/AGCCTTGTGCCATGGACATGTCTACT/BHQ_1/-3′ | 200 nM | |
Bmal1 (NM_001243048) | Forward: 5′-AGCTTCTGCACAATCCACAGCAC-3′ | 300 nM |
Reverse: 5′-TGTCTGGCTCATTGTCTTCGTCCA-3′ | 300 nM | |
Probe:5′-/5HEX/-AAAGCTGGCCACCCACGAAGATGGG/BHQ_1–3′ | 200 nM | |
Opn4 (NM_001128599.1) | Forward: 5′-ACATCTTCATCTTCAGGGCCA-3′ | 300 nM |
Reverse: 5′-ACTCACCGCAGCCCTCAC-3′ | 300 nM | |
Rpl37a (NM_009084.4) | Forward: GCATGAAAACAGTGGCCGGT | 300 nM |
Reverse: CAGGGTCACACAGTATGTCTCAAAA | 300 nM | |
18S RNA | Forward: 5′-CGGCTACCACATCCAAGGAA-3′ | 50 nM |
Reverse: 5′-GCTGGAATTACCGCGGCT-3′ | 50 nM |
