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Figure 1

From: Engineering a short-chain dehydrogenase/reductase for the stereoselective production of (2S,3R,4S)-4-hydroxyisoleucine with three asymmetric centers

Figure 1

The enzymatic reaction of HILDH and 4-HIL stereoisomers production. (a) Schematic diagram of the enzymatic reaction of HILDH. HILDH reduces AMKP to 4-HIL in an NADH-dependent manner. Asymmetric centers are shown in asterisks. (b) HPLC chromatogram of 4-HIL stereoisomers produced (left) and their production ratios (right). The reaction mixtures were derivatized with GITC and detected by HPLC. Each peak label on the chromatogram (left) corresponds to each 4-HIL stereoisomer listed on the right. “No enzyme” indicates the reaction mixtures without HILDH.

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