Figure 5 | Scientific Reports

Figure 5

From: Engineering a short-chain dehydrogenase/reductase for the stereoselective production of (2S,3R,4S)-4-hydroxyisoleucine with three asymmetric centers

Figure 5

HPLC analysis of 4-HIL stereoisomers produced by the wild-type (WT) and the mutant HILDH. (a) HPLC chromatogram (left) and bar graph (right) of 4-HIL stereoisomers produced by the wild-type HILDH and the mutants of R88, R147 and Y191. “No enzyme” represents the reaction mixtures without HILDH. The reaction mixtures were derivatized with GITC and detected by HPLC. For all rows, dotted line in bar graph divides products into 4S forms (below the dotted line) and 4R forms (above the dotted line) of 4-HIL. (b) 4-HIL stereoisomers produced by the R88 mutants. ND means not detected by HPLC analysis. Dotted line divides products into 4S forms (below the dotted line) and 4R forms (above the dotted line) of 4-HIL. (c) 4-HIL stereoisomers produced by the W242 mutants. ND means not detected by HPLC analysis. Dotted line divides products into 4S forms (below the dotted line) and 4R forms (above the dotted line) of 4-HIL.

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