Figure 3

Treg cells (FOXP3⁺ lymphocytes) cell count. Treg cells were stained in brown, and the total area occupied by these cells was measured. Sham animals presented no Treg cells; I + PY group showed a higher infiltration of FOXP3⁺ cells within the infarcted and peri-infarcted zone when compared to I groups (P = 0.007, Kruskal-Wallis test). As expected, I group and I + PY were statistically different from the Sham group (P < 0.0001, Kruskal-Wallis test). Values are expressed as mean ± SEM. (Immunohistochemistry, DAB, scale bar 400 μm).