Figure 3 | Scientific Reports

Figure 3

From: Photosensing and Thermosensing by Phytochrome B Require Both Proximal and Distal Allosteric Features within the Dimeric Photoreceptor

Figure 3

Spectroscopic properties of PSM mutants impacting the N-terminal extension (NTE) in PhyB. (a) Assembly of NTE truncations with PΦB. Purified PSM fragments were subjected to SDS-PAGE and either stained for protein with Coomassie Blue or for the bound bilin by zinc-induced fluorescence. For full-length images of the SDS-PAGE gels see Supplementary Fig. S1. (b) UV-Vis absorption and difference spectra of dark-adapted samples (Pr, red) and samples irradiated to steady state with 230 μmol⋅m−2⋅s−1 of 630-nm red light (RL, purple). The difference spectra are shown at 70% total amplitude. The absorption and difference maxima/minima are indicated.

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