Figure 8
Assay for suppression of BAX-triggered cell death (BT-PCD) by the candidate Heterodera avenae effectors (a) c68622.graph_c0 and (b) c72543.graph_c0 in Nicotiana benthamiana. Leaves of N. benthamiana were infiltrated with the infiltration buffer or Agrobacterium tumefaciens cells containing a pGR107 vector carrying the candidate effector gene either alone or infiltration with A. tumefaciens cells carrying a mouse Bax gene 24 h later. Photos of the phenotypes of infiltrated N. benthamiana leaves were taken 6 days after infiltration. The spots with label 3 on the leaf show that the gene c68622.graph_c0 suppressed the necrosis induced by Bax, but the gene c72543.graph_c0 did not suppress necrosis.
