Table 2 Validation of mRNA-Seq data of a selected set of wheat and Heterodera avenae genes with quantitative real-time PCR (qPCR) to confirm gene expression changes at the contact stage (treatment versus control).

From: Transcriptional responses of wheat and the cereal cyst nematode Heterodera avenae during their early contact stage

Gene ID

Annotation

Log2FC

qPCR

mRNA-seq

Wheat DEGs

gene:TRAES3BF074000020CFD_g

Unnamed protein product [Triticum aestivum]

0.01

Inf (up)*

gene:Traes_1AS_F9013A945a

Phenylalanine ammonia-lyase [Aegilops tauschii]

−0.76*

−1.17*

gene:Traes_2AL_8394449B2

Ubiquinol oxidase 1, mitochondrial (Precursor) [Nicotiana tabacum]

−1.19*

−1.27*

gene:Traes_2AS_EE549925C

Root peroxidase [T. aestivum]

−0.36

−0.67*

gene:Traes_3DL_EE0699FDC

Secologanin synthase [Ae. tauschii]

2.50*

2.80*

gene:Traes_6DS_768787FF4a

Auxin-induced protein [Ae. tauschii]

−0.83*

−1.31*

gene:Traes_5bs_bcc1b9791a

Respiratory burst oxidase homolog protein B [Oryza sativa subsp. japonica]

0.70*

0.73*

gene:Traes_4bl_eb96605eda

Agmatine coumaroyltransferase-2 [Hordeum vulgare]

−0.89*

−0.76*

gene:Traes_4al_dd83f1a44a

Xylanase inhibitor [T. aestivum]

−0.92*

−0.71*

gene:Traes_2al_1a870ce7ba

Probable aldo-keto reductase 3 [O. sativa subsp. japonica]

−0.56*

−0.61*

gene:Traes_4bs_63dd9d036a

Lipoxygenase [T. aestivum]

0.21

−0.67*

gene:Traes_1bl_04b591073a

NEP1-interacting protein 2 [Arabidopsis thaliana]

0.27

1.07*

Nematode DEGs

c73395.graph_c0

Macrophage migration inhibitory protein [Eriocheir sinensis]

−0.91

−2.00*

c62312.graph_c0

Programmed cell death protein 2 [Toxocara canis]

−2.32*

−1.05*

c73973.graph_c0

Neprilysin-2 [T. canis]

1.15*

1.08*

c78521.graph_c0

Fatty acyl-CoA desaturase, putative [Eimeria tenella]

2.42*

1.16*

c54125.graph_c0

Sialin [Ascaris suum]

1.99*

1.75*

c72543.graph_c0b

Pectate lyase [Heterodera glycines]

2.17*

1.94*

c76930.graph_c0

Putative salivary protein [Culicoides sonorensis]

6.49*

2.09*

c79218.graph_c0

Predicted: transmembrane BAX inhibitor motif-containing protein 4-like [Amphimedon queenslandica]

Inf (up)

2.46*

c68622.graph_c0b

Chitinase [H. glycines]

3.60*

3.32*

c78853.graph_c0b

Cathepsin L2 [Sinonovacula constricta]

0.77*

1.83*

  1. Inf (up) indicates that the expression of the gene was detected only in the treatment sample, but not the control sample. *P < 0.05. FC, fold change (treatment vs. control). aThe DEGs were involved in biotic stress pathways of wheat created using MapMan visualization. bThe DEGs were predicted effector genes in Heterodera avenae exposed to wheat roots.
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