Figure 2 | Scientific Reports

Figure 2

From: microRNA-181b is increased in cystic fibrosis cells and impairs lipoxin A4 receptor-dependent mechanisms of inflammation resolution and antimicrobial defense

Figure 2

miR-181b is overexpressed in CF airway cells and controls ALX/FPR2 expression and signaling. (A) miR-181b levels were evaluated by real-time PCR in normal (16HBE14o-), CF (CFBE41o-) and normalized CF (CFBE41o- CEP-CFTR wt 62 kb) epithelial respiratory cells. RNU6 and SNORD95 were used for normalization. Bars are mean ± SEM from n = 3. *p = 0.04 vs CFBE41o-; **p = 0.02 vs 16HBE14o-. (B) miR-181b levels in primary bronchial epithelial cells from 3 non-CF subjects and 3 CF patients carrying the ΔF508/ΔF508 mutation. Bars are mean ± SEM; **p = 0.0027 (C) CFBE41o- were transfected with a miR-181b inhibitor. miR-181b expression was evaluated by real-time PCR 24 h post transfection. Results are mean ± SEM from 3 separate transfections. ***p = 0.0001. (D) Total ALX/FPR2 protein expression was evaluated by flow cytometry in CFBE41o-cells, transfected with either a negative control or a miR-181b inhibitor. ALX/FPR2 levels were evaluated 48 h post-transfection. Bars depict mean ± SEM from 3 separate transfections. ***p = 0.0005. The inset shows a representative histogram. (E) CFBE41o- were transfected with a negative control or a miR-181b inhibitor. Transepithelial electrical resistance (TEER) was measured 48 h post-transfection using a EVOM Voltohmmeter. Results are expressed as mean ± SEM from 3 separate transfections. **p = 0.0026 vs CTRL.

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