Figure 6
The protective effect of hydrogen gas at different concentrations in vitro. TUNEL staining, flow cytometry, and ROS detection were conducted to identify the optimal dose of H2 (A). The number of apoptotic cells decreased markedly after H2 treatment; significant differences were also noted among the different H2 groups and the largest reductions were observed in the H/R + 75% H2 group (P < 0.05). *P < 0.05 vs. control; # P < 0.05 vs. H/R; § P < 0.05 vs. H/R + 12.5% H2; ‖ P < 0.05 vs. H/R + 25% H2; & P < 0.05 vs. H/R + 50% H2.
