Figure 8

The roles of Akt1 and Akt2 in the protective effects of 75% H2. Western blotting was performed to determine the protein expression of p-Akt1 (Ser 473), Akt1, p-Akt2 (Ser 474), Akt2, p-Akt (Thr 308), p-Akt (Thr 450), Akt, p-GSK-3β, GSK-3β, pro-caspase-3, and cleaved caspase-3 (A,B). *P < 0.05 vs. control; # P < 0.05 vs. H/R; § P < 0.05 vs. H/R + HCH; ‖ P < 0.05 vs. H/R + HCH + A; & P < 0.05 vs. H/R + HCH + C.