Table 1 ANOVAs on the effects of pH (ambient and low pH) and nutrient enrichment (control, moderate and high) on the leaf content of chlorophyll a, chlorophyll b, carotenoids, the leaf growth and specific leaf growth. Analyses of the percentage cover of encrusting coralline algae, non-encrusting algae and total epiphytic assemblages on P. oceanica leaves also include the factor plot (3 levels, random and nested within the interaction pH × Nutrients). *P < 0.05; **P < 0.01; ***P < 0.001. SNK tests are included for significant factors or “pH × Nu” interaction.
From: Nutrient Loading Fosters Seagrass Productivity Under Ocean Acidification
df | MS | F | ||
|---|---|---|---|---|
Chlorophyll a | ||||
pH | 1 | 7.746 | 7.440** | |
Nutrient (Nu) | 2 | 1.279 | 1.228 | |
pH × Nu | 2 | 1.848 | 1.775 | |
Residual | 12 | 1.041 | ||
Chlorophyll b I | ||||
pH | 1 | 0.192 | 12.096** | |
Nutrient (Nu) | 2 | 0.032 | 2.050 | |
pH × Nu | 2 | 0.035 | 2.221 | |
Residual | 12 | 0.016 | ||
Carotenoids | ||||
pH | 1 | 4.007 | 11.788** | |
Nutrient (Nu) | 2 | 0.491 | 1.443 | |
pH × Nu | 2 | 0.483 | 1.421 | |
Residual | 12 | 0.34 | ||
Leaf growth | SNK tests | |||
pH | 1 | 0.841 | 1.247 | Control nutrient: ambient pH > low pH |
Nutrient (Nu) | 2 | 0.479 | 0.710 | Moderate nutrient: ambient pH = low pH |
pH × Nu | 2 | 2.801 | 4.156* | High nutrient: ambient pH = low pH |
Residual | 12 | 0.674 | ||
Specific leaf growth II | SNK tests | |||
pH | 1 | 0.265 | 51.776*** | dAmbient pH: control = moderate = high |
Nutrient (Nu) | 1 | 0.032 | 6.232** | Low pH: control = moderate < high |
pH × Nu | 2 | 0.019 | d3.628* | |
Residual | 12 | 0.005 | ||
Encrusting coralline algae | SNK tests | |||
pH | 1 | 48014 | 230.602*** | eAmbient pH: control < moderate = high |
Nutrient (Nu) | 2 | 1584 | 7.606** | Low pH: control = moderate = high |
pH × Nu | 2 | 1436 | e6.896** | |
Plot (pH × Nu) | 12 | 208 | 2.877** | |
Residual | 36 | 72 | ||
Total non-calcified algae II | ||||
pH | 1 | 35.876 | f5.561* | fpH: ambient > low |
Nutrient (Nu) | 2 | 36.408 | g5.643** | gNutrient: control < moderate = high |
pH × Nu | 2 | 17.907 | 2.775 | |
Plot (pH × Nu) | 12 | 6.452 | 3.032** | |
Residual | 36 | 2.128 | ||
Total epiphytic assemblage I | ||||
pH | 1 | 1.473 | h38.499*** | hpH: ambient > low |
Nutrient (Nu) | 2 | 0.450 | i11.774** | iNutrient: control < moderate = high |
Ph × Nu | 2 | 0.048 | 1.257 | |
Plot (pH × Nu) | 12 | 0.038 | 1.570 | |
Residual | 36 | 0.024 | ||