Table 1 ANOVAs on the effects of pH (ambient and low pH) and nutrient enrichment (control, moderate and high) on the leaf content of chlorophyll a, chlorophyll b, carotenoids, the leaf growth and specific leaf growth. Analyses of the percentage cover of encrusting coralline algae, non-encrusting algae and total epiphytic assemblages on P. oceanica leaves also include the factor plot (3 levels, random and nested within the interaction pH × Nutrients). *P < 0.05; **P < 0.01; ***P < 0.001. SNK tests are included for significant factors or “pH × Nu” interaction.

From: Nutrient Loading Fosters Seagrass Productivity Under Ocean Acidification

 

df

MS

F

 

Chlorophyll a

pH

1

7.746

7.440**

 

Nutrient (Nu)

2

1.279

1.228

 

pH × Nu

2

1.848

1.775

 

Residual

12

1.041

  

Chlorophyll b I

pH

1

0.192

12.096**

 

Nutrient (Nu)

2

0.032

2.050

 

pH × Nu

2

0.035

2.221

 

Residual

12

0.016

  

Carotenoids

pH

1

4.007

11.788**

 

Nutrient (Nu)

2

0.491

1.443

 

pH × Nu

2

0.483

1.421

 

Residual

12

0.34

  

Leaf growth

   

SNK tests

pH

1

0.841

1.247

Control nutrient: ambient pH > low pH

Nutrient (Nu)

2

0.479

0.710

Moderate nutrient: ambient pH = low pH

pH × Nu

2

2.801

4.156*

High nutrient: ambient pH = low pH

Residual

12

0.674

  

Specific leaf growth II

   

SNK tests

pH

1

0.265

51.776***

dAmbient pH: control = moderate = high

Nutrient (Nu)

1

0.032

6.232**

Low pH: control = moderate < high

pH × Nu

2

0.019

d3.628*

 

Residual

12

0.005

  

Encrusting coralline algae

   

SNK tests

pH

1

48014

230.602***

eAmbient pH: control < moderate = high

Nutrient (Nu)

2

1584

7.606**

Low pH: control = moderate = high

pH × Nu

2

1436

e6.896**

 

Plot (pH × Nu)

12

208

2.877**

 

Residual

36

72

  

Total non-calcified algae II

    

pH

1

35.876

f5.561*

fpH: ambient > low

Nutrient (Nu)

2

36.408

g5.643**

gNutrient: control < moderate = high

pH × Nu

2

17.907

2.775

 

Plot (pH × Nu)

12

6.452

3.032**

 

Residual

36

2.128

  

Total epiphytic assemblage I

pH

1

1.473

h38.499***

hpH: ambient > low

Nutrient (Nu)

2

0.450

i11.774**

iNutrient: control < moderate = high

Ph × Nu

2

0.048

1.257

 

Plot (pH × Nu)

12

0.038

1.570

 

Residual

36

0.024

  
  1. IData log (x + 1) transformed to satisfy parametric test assumptions.
  2. IIData sqrt (x + 1) transformed to satisfy parametric test assumptions.