Figure 1
From: Profiling of miRNAs and target genes related to cystogenesis in ADPKD mouse models
Characterization of mice with kidney collecting duct-specific Pkd1 knockout. (A) The whole kidney sections from mice at postnatal day 1 (P1), P3, and P7 were stained with the collecting duct-specific marker Dolichos biflorus agglutinin (DBA, red) and the proximal tubule marker Lotus tetragonolobus lectin (LTL, green). Nuclei were counterstained with DAPI (blue). (B) The areas of cysts derived from collecting ducts were measured and classified by cyst sizes: non-cystic area, dilated tubules (<1 × 105 μm2), small cysts (1 × 105–106 μm2), and large cysts (>1 × 106 μm2). (C) Kidney/body weight ratio and (D) serum urea nitrogen (BUN) concentration in Pkd1 f/f versus Pkd1 f/f:HoxB7-Cre mice. Data are shown as mean ± SD; n ≥ 10 for each time point; *P < 0.05; **P < 0.01; ***P < 0.001. (E) Survival rate curves of Pkd1 f/f versus Pkd1 f/f:HoxB7-Cre mice. n ≥ 10.
