Figure 6

Venn diagram of binding epitopes and structure models of CD38 in complex with nanobodies and mAbs. (a) Venn diagram of binding epitopes on CD38 for nanobodies and the single chain variable fragment of daratumumab. Numbers indicate family numbers of CD38-specific nanobodies. (b) 3D-structures of CD38 (yellow) in complex with NAD+ (orange), nanobodies MU375 (cyan, family 5), MU551 (grey, family 20), or the Fab fragment of mAb hb7 (red) or of mAb sar650984 (blue) were aligned with PyMOL using PDB-IDs 2i65, 5f21, 5f10, 3raj, and 4cmh, respectively. Mutation of serine 274 (green) to phenylalanine abolishes binding of daratumumab.