Table 1 Characteristics of CD38-specific nanobody families.

From: Nanobodies effectively modulate the enzymatic activity of CD38 and allow specific imaging of CD38+ tumors in mouse models in vivo

family

llama

isolate

variant

max diff

CDR3

name

clone

Kdis s −1

epitope

1

538

3

2

1

AHTFSGSF

l-8.1b

WF9

2.2E-03

3

2

539

6

6

10

DHTFAGVY

l-8.2a

JK36

2.0E-04

3

3

539

1

1

0

VIRTYSTY

l-8.3a

WF42

3.8E-04

3

4

538

4

4

8

WHYAAGRDY

l-9.1c

JK2

1.1E-03

2

5

25

4

2

11

ASTAVGADT

l-9.2a

MU370

5.1E-04

1

6

538

2

2

8

GNPGTRYIY

l-9.3b

JK29

1.8E-04

1

7

538

2

2

3

DRFSVVAVEYDY

l-12b

WF14

6.3E-03

3

8

539

3

3

2

GLKRIGDQREADY

l-13a

JK28

6.3E-04

1

9

10

13

5

14

DRFVVAAGTHDLDY

s-14a

MU738

2.9E-03

1

10

538

1

1

0

DRFTLVPTTSDLDY

l-14.1a

JK44

2.4E-04

1

11

539

4

4

7

RFWIGVRAPAEYNY

l-14.2b

JK22

3.2E-03

1

12

25

3

3

39

DRLVLVALSIADPGF

l-15.1b

MU1068

2.1E-04

1

13

25

27

2

21

GFPVLVALSIADPDY

l-15.2a

MU274

1.1E-04

1

14

10

9

1

0

GRGIVAGRIPAEYAD

l-15.3a

MU1053

9.0E-04

1

15

539

1

1

0

GRTASASTMIREYDS

l-15.4a

JK19

1.1E-04

3

16

10

9

2

2

TTSVVVLLAPNWYEY

l+/−15a

MU415

1.1E-03

1

17

538

1

1

0

RLRGWITTRKPNEYDY

s-16a

WF211

4.5E-03

1

18

25

4

3

13

ARSAGLGSSRRIEGYDK

l-17a

WF121

2.3E-04

3

19

25

9

2

20

QYQDRYYDEFTWKEKDMDY

l-19.1a

MU523

7.8E-05

2

20

25

3

2

6

RYQPRYYDSGDMDGYEYEF

l-19.2a

MU1067

1.2E-04

2

21

25

2

2

14

ADRFRGWATWRDDPDQYDY

s-19b

WF139

2.2E-04

3

22

10

6

6

13

DVTLNPFTGWDTRSGPMYRYEYDY

s+/−24a

WF100

5.1E-04

3

Daratumumab

VL: RSN

VH: DKILWFGEPVFDY

c-13

Dara scFv

4.4E-03

1

  1. Families were designated in order of increasing CDR3 lengths. Isolate indicates the number of clones selected per family, variant indicates the number of clones carrying distinct but evidently related amino acid sequences, max diff indicates the maximum difference between two members of a family in number of amino acid substitutions. Variant amino acid positions in the CDR3 within a family are indicated in italic. Names indicate the presence of a short (s) or long hinge (l), the absence (−) or presence (+) of a disulfide bond connecting CDR2 and CDR3, and the length of the CDR3 in numbers of amino acid residues. Kdis shows off-rates determined by SPR analyses on the immobilized glycosylated extracellular domain of CD38. Epitopes are numbered arbitrarily, with nanobodies that block the binding of one another considered to recognize the same or overlapping epitopes.
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