Figure 2 | Scientific Reports

Figure 2

From: Improved detection of BRAF V600E using allele-specific PCR coupled with external and internal controllers

Figure 2

Screening ASP using tAS-PCR with gradient Tm. The C q values (i.e., left Y-axis) of WT- or MT-gDNA at a final concentration of 50 ng and their corresponding ΔC q values (i.e., right Y-axis) in tAS-PCR system using ASP oligonucleotide of HQ-663 under a gradient Tm ranging from 60 °C to 67 °C, as indicated on the X-axis. The results of HQ-666 and -668 show similar patterns to that of abovementioned HQ-663 (data not shown). The standard deviation (SD) values of the cycle numbers are indicated as error bars. For the reaction mixture that did not show positive amplification signal in 55 thermal cycles, the C q values were recorded as 55 to simplify the analysis. At an optimal Tm of 60 °C, the average C q values of V600E MT-alleles in the tAS-PCR system using HQ-668, -666, and -663 were 26.25 ± 0.24, 30.79 ± 0.52, and 28.95 ± 0.50, respectively.

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