Figure 1

Overexpression of TFPI-2 in MDA231 cells suppresses cell invasion and migration. (A) Western blots showing the expression of TFPI-2 protein in MDA231/TFPI-2 and MDA231/con cells with an anti-His antibody. (B) Upper panel, expression of TFPI-2 mRNA was analyzed by qRT-PCR in TFPI-2 knockdown cells. *P < 0.05 as determined by one-way ANOVA followed by Tukey’s multiple comparison tests. Lower panel, MDA231 clones were subjected to western blots for the expression of TFPI-2 using antibodies against TFPI-2 and β–actin. (C) MTT assays indicate that overexpression of TFPI-2 decreased proliferation of MDA231 cells. Bars indicate standard error of mean from three independent experiments. P < 0.05 as determined by Student’s t-test. (D) Representative images and statistical analyses of transwell assays indicate the invasive capacity of MDA/TFPI-2 vs. MDA231/con cells and MDA231/Sh2 vs. MDA231/sh-con cells. **P < 0.01 as determined by Student’s t-test. (E) Representative micrographs of wound healing assay of the indicated cells. Wound closures were photographed at 0 and 36 hours after wounding. Statistical analysis was assessed from three independent experiments; ± SD. *P < 0.05 as determined by Student’s t-test.