Figure 3
From: Localization of TFPI-2 in the nucleus modulates MMP-2 gene expression in breast cancer cells
Down-regulation of MMP-2 expression in MDA231/TFPI-2 cells. (A) qRT-PCR was performed to analyze the relative levels of MMP-2 mRNA in MDA/TFPI-2 vs. MDA231/con cells and MDA231/Sh2 vs. MDA231/sh-con cells. Bars indicate the standard error of mean from three independent experiments, *P < 0.05 as determined by Student’s t-test. (B) Western blots indicate the expression of MMP-2 protein in MDA231/TFPI-2 and MDA231/con cells. (C) ELISA assays were used to measure the secreted levels of MMP-2 protein in culture medium of MDA231 cell clones. (D) Upper panel, a representative gelatin zymography showing the enzymatic activity of MMP-2 in the culture medium of MDA231 cell lines. Lower panel, the intensity of the bands were assessed by densitometric semi-quantitation from three independent experiments and depicted by a bar diagram, ± SD. *P < 0.05 as determined by one-way ANOVA followed by Tukey’s multiple comparison tests.
