Figure 5 | Scientific Reports

Figure 5

From: Seed defense biopriming with bacterial cyclodipeptides triggers immunity in cucumber and pepper

Figure 5

Translational approach for seed defense biopriming of pepper plants with PB69 ACM under field conditions. (a) Disease severity of pepper plants subjected to SDB with PB69 ACM was assessed 7 days after infection with P. syringae pv. lachrymans at 60, 70, and 80 dps. (b) Shoot length was assessed every 10 dps during 60−110 dps. (c) Fruit yield of pepper plants subjected to SDB with PB69 ACM was assessed at 130 and 150 dps. (df) Expression levels of the cucumber resistance genes CaLOX (two-way ANOVA, n = 3 experiments, ***P < 0.001, F(7,16) = 7.96) (d), CaPR1 (two-way ANOVA, n = 3 experiments, ***P < 0.001, F(7,16) = 14.45) (e), and CaChi2 (two-way ANOVA, n = 3 experiments, ***P < 0.001, F(7,16) = 3.67) (f) were assessed by qRT-PCR at 0 and 6 h after infiltrating pepper with X. axonopodis pv. vesicatoria. Water and 1 mM BTH were used as negative and positive controls, respectively. Different letters indicate significant differences between treatments (P = 0.05 according to least significant difference). An asterisk (***) indicates a significant difference (P < 0.001). Error bars indicate the standard error (n = 30).

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