Figure 4

Effect of whey protein concentrate (WPC), rapamycin, and their combination on the expression of Nrf2, GCLC, and glutathione reductase (GR) proteins in MDA-MB-231 cells. Representative western blots showing the effect of 48 hours of exposure to 10 mg/mL WPC, 1 μM rapamycin, and their combination on whole cell Nrf2, GCLC, and GR protein levels. β-actin was used as a loading control. Data represent the means ± SD from three experiments.