Figure 6 | Scientific Reports

Figure 6

From: Whey Protein Concentrate Renders MDA-MB-231 Cells Sensitive to Rapamycin by Altering Cellular Redox State and Activating GSK3β/mTOR Signaling

Figure 6

Effect of whey protein concentrate (WPC), rapamycin, and their combination on the mTOR/GSK3β signaling pathway in MDA-MB-231 cells. Representative western blots showing levels of p-mTOR (Ser 2448), mTOR, p-p70S6K (Thr 389), p70S6K, p-4E-BP-1, 4E-BP-1, p-GSK3β, and GSK3β in cells treated with 10 mg/mL WPC, 1 μM rapamycin, or both for 48 hours. β-actin (1) was used as a loading control of p-mTOR (Ser 2448), mTOR, p-p70S6K (Thr 389), p70S6K, p-4E-BP-1, and 4E-BP-1. β-actin (2) was used as a loading control of p-GSK3β, and GSK3β Data represent the means ± SD from three experiments.

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