Figure 1 | Scientific Reports

Figure 1

From: Nucleotide and receptor density modulate binding of bacterial division FtsZ protein to ZipA containing lipid-coated microbeads

Figure 1

FtsZ binding to sZipA immobilized in microbeads. (a) Scheme of the experimental procedure followed for determination of FtsZ binding to ZipA immobilized in lipid coated microbeads. Concentration of bead-associated FtsZ-GDP (b) and FtsZ-GTP (c) plotted as a function of the concentration of free FtsZ-GDP and FtsZ-GTP, respectively, for different densities of immobilized ZipA, proportional to the percentage of total lipid bearing NTA (in the legend) coating a 60 g/l suspension of 5 micron diameter beads. Symbols are the data. Dotted lines are only meant to guide the eye. The horizontal dashed line represents the amount of FtsZ that would completely cover the bead as a monolayer, calculated as described in the text.

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