Table 1 Different types of MSAP within the levels of methylated cytosine for Valencia Orange/Rangpur lime (VO/RL) and Valencia Orange/Sunki Maravilha (VO/SM) exposed to full irrigation (Control) and to the severe conditions of recurrent water deficit (WD1, WD2 and WD3).

From: Recurrent water deficit causes epigenetic and hormonal changes in citrus plants

MSAP band typea

VO/RL

VO/SM

CONTROL

WD1

WD2

WD3

CONTROL

WD1

WD2

WD3

I

93

122

121

127

156

117

111

117

II

97

29

41

28

27

30

52

52

III

70

45

49

52

38

71

56

52

IV

62

126

111

115

88

91

90

88

Total amplified bands

322

322

322

322

309

309

309

309

Total methylated bandsb

229

200

201

195

153

192

198

192

Fully methylated bandsc

132

171

160

167

126

162

146

140

MSAP%d

71.1

62.1

62.4

60.6

49.5

62.1

64.1

62.1

Hemi-methylated ratio(%)e

30.12

9.01

12.73

8.70

8.74

9.71

16.83

16.83

Fully methylated ratio (%)f

40.99

53.11

49.69

51.86

40.78

52.43

47.25

45.31

  1. aType I is the presence of bands in both EcoRI/HpaII and EcoRI/MspI enzymes and indicates the absence of methylation; type II are bands generated in EcoRI/HpaII enzymes but not in EcoRI/MspI enzymes; type III bands appear in EcoRI/MspI enzymes but not in EcoRI/HpaII enzymes; and type IV represents the absence of band in both enzyme combinations.
  2. bTotal methylated bands are II + III + IV bands.
  3. cFully methylated are III + IV bands.
  4. dMSAP is a percentage ratio of total methylated bands (II + III + IV) to total amplified bands.
  5. eHemi-methylated ratio is a percentage ratio of total hemi-methylated bands (II) to total amplified bands.
  6. fFully methylated ratio is a percentage ratio of fully methylated bands (III + IV) to total amplified bands.
Back to article page