Figure 7

The effects of AMD3100, PD98059, and SDF-1α on FIR-preconditioned BMSC proliferation and migration. (A) Confocal images of Hoechst33342-stained BMSCs^con and BMSCs^{FIR 50 min} pre-treated with one or both of these inhibitors. These cells were pretreated for 2 h, then the FIR 50 min was performed, and all cells incubated for 72 h. Scale bars are 10 μm. Graph indicating the proliferation of these cells with one or both of these inhibitors, as identified using an EZ-Cytox assay. (B) Phase contrast images of transwell migration assay for BMSCs^con and BMSCs^{FIR 50 min} with one or both of these inhibitors. Graph indicating the rate of cell migration. (C) Time lapse images of BMSCs^con and BMSCs^{FIR 50 min} with and without pretreatment of SDF-1α (20 ng/ml). Graph of proliferation rates, using a cell counter at the indicated time points for representative samples, shown as percentage increases compared to BMSCs^con at t = 24 h. All data represent the mean ± SD of three independent experiments in triplicate assays expressed as percentages of the BMSCs^con. For all pretreatments *P < 0.05, **P < 0.01, ***P < 0.001 versus BMSCs^con. (−, untreated; +, FIR^{50 min} preconditioning).