Table 2 Percentage of tumour cells in various organs from control (C1-5) and KPT-330 treated (K1-5) SCID mice.

From: Cytoplasmic cyclin D1 controls the migration and invasiveness of mantle lymphoma cells

Mouse

Organ

% of CD20+ cells

% of CD45+ cells

C1

Brain

<1

<1

BM

33

34

Spleen

61

64

Blood

3

nda

C2

Brain

<1

<1

BM

22

21

Spleen

62

65

Blood

11

nd

C3

Brain

2

2

BM

53

60

Spleen

78

79

Blood

5

nd

C4

Brain

2

1

BM

62

63

Spleen

78

79

Blood

4

nd

C5

Brain

7

4

BM

11

10

Spleen

nd

nd

Blood

<1

1.0

K1

Brain

0

0

BM

<1

<1

Spleen

3

<1

Blood

<1

<1

K2

Brain

1

1

BM

<1

<1

Spleen

<1

<1

Blood

<1

<1

K3

Brain

2

3

BM

<1

<1

Spleen

<1

<1

Blood

<1

<1

K4

Brain

<1

<1

BM

<1

<1

Spleen

<1

<1

Blood

<1

<1

K5

Brain

5

6

BM

<1

<1

Spleen

<1

<1

Blood

<1

<1

  1. JeKo1-mCherry-Luc cells were injected into the caudal vein of 10 SCID mice, which were then randomised into two groups: a control group (C series) and a group receiving KPT-330 (K series). K1-5 mice were treated orally, twice weekly, with 1 mg/kg KPT-330. The overall distribution of MCL tumor cells was determined by BLI during the time course of the experiment and analysed by flow cytometry at the time of mouse death (day 35 after cells injection for all mice except C5, which was killed on day 28). Cells were isolated from the organs indicated and stained with CD20-PE or CD45-VioBlue Abs. The percentage of stained cells was deduced from the cytometry profiles analyzed with the Kaluza software v1.5 A. and, not determined.
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