Figure 1 | Scientific Reports

Figure 1

From: Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures

Figure 1

Decrease of AKAP350 expression induces defective lumen formation. AKAP350KD MDCK cells and control cells expressing non-specific small hairpin (sh)RNAs were generated as described in Materials and Methods. (a) Immunoblots showing AKAP350 expression in control and AKAP350KD cells and the corresponding loading control. (b–e) Control and AKAP350KD cells were grown in Matrigel for 72 h, stained and analyzed by confocal microscopy. Single confocal sections at the center of the cysts are shown. Merged images show AKAP350 (b), actin (c) and ocludin or γ-tubulin (e) staining in red and DAPI staining in blue in control and AKAP350KD cysts. (d) Bars represent the percentage of cysts that developed each lumen phenotype for at least 10 cysts analyzed in 6 independent experiments. Scale bars, 10 μm. *p < 0.05; **p < 0.01.

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