Figure 7 | Scientific Reports

Figure 7

From: Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures

Figure 7

Interference with AKAP350/EB1 interaction is sufficient to induce defective cystogenesis. (a) Images show EB1 (green) and γ-tubulin (red) staining in control and AKAP350NTD mitotic cells. (b) Control and AKAP350NTD cells were grown in Matrigel for 72 h, stained and analyzed by confocal microscopy. Single confocal sections at the center of the cysts are shown. Merged images show EB1 (green), α-tubulin (red) and DAPI (blue) staining in control and AKAP350NTD cells. Bars represent the percentage of cysts that developed each type of phenotype for at least 8 cysts analyzed in 4 independent experiments. Scale bars, 10 μm. *p < 0.05.

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