Table 1 The effects of the mutated residue on the Kd or EC50 value loss compared with those of wild-type P2Y1R.

From: Identification of a Different Agonist-Binding Site and Activation Mechanism of the Human P2Y1 Receptor

Mutated residue

Position

Kd or EC50 value loss

Reference

Y110F

2.63

NS

17

R128A

3.29

>100000

38

H132A

3.33

13

38

Y136A

3.37

10

38

T205A

ECL2

NS

17

T221A

5.42

13

38

T222A

5.43

9

38

K280A

6.55

810

6

N283A

6.58

NS

17

R287A

6.62

11654

38

Y303F

7.32

1.24

17

Y306F

7.35

>300

17

R310A

7.39

329

38

  1. NS: negligible specific binding for [3H]2MeSADP in the mutant P2Y1R.
Back to article page