Figure 5

HFD induces the increase of Vγ4⁺ γδ T cells in the skin draining lymph node and blood. (a–c) Ear swelling response in IMQ-induced psoriasis model (a), body weight (b) and the number of Vγ4⁺ γδ T cells in the whole ear skin and the draining LNs (c) of steady state ND- and HFD-fed mice at 1 and 3 week after HFD-feeding. (d) Flow cytometric analysis of the number of Vγ4⁺ γδ T cells in the whole ear skin, cervical lymph node and peripheral blood of steady state ND- and HFD-fed mice at 10 week after HFD-feeding. Results are expressed as the mean ± SD (a), SEM (b–d). p-values were obtained by Mann-Whitney-U-test. *p ≤ 0.05. Data are from one experiment, representative of independent two experiments with three to four mice (a–c), two experiments with two to three mice (d).