Figure 3
From: Baf53a is involved in survival of mouse ES cells, which can be compensated by Baf53b
Expression of undifferentiated state marker genes in Baf53a-deficient ES cells. (A) Protein expression of markers characteristic of the undifferentiated state was analyzed by Western blot in tetracycline (Tet)-inducible Baf53a conditional knockout (cKO) ES cells. Baf53a cKO ES cells were cultured with or without 1 μg/mL Tet for 2–4 days. αTubulin was used as a loading control. The data is representative of three independent experiments. (B) Expression of Dax1, Oct3/4, Esrrb, and Nanog mRNA was examined by quantitative RT-PCR. The data was normalized to GAPDH expression, and the results are the means ± standard deviations of three independent experiments. *p < 0.05 (t-test).
