Figure 6
From: Baf53a is involved in survival of mouse ES cells, which can be compensated by Baf53b
Expression of Baf53b rescues the survival of Baf53a-deficient ES cells. (A) Morphological signatures of Baf53b-expressing Baf53a cKO ES cells. Baf53a cKO ES cells were transfected with expression vectors for empty (ev), Baf53a, or Baf53b. Transfected ES cells were divided into two dishes at 48 h after transfection and cultured with or without 1 μg/mL Tet. Cell morphologies were observed after 6 days. Scale bar is 250 μm. (B,C) Colony formation assay. (B) Control and Baf53a- or Baf53b-expressing Baf53a cKO ES cells as described in panel A were cultured for 6 days with or without 1 μg/mL Tet; and these samples were stained with crystal violet. (C) The colonies were dissolved in 1.0% SDS solution and absorbance was measured at 600 nm to quantify the number of surviving colonies. The representative results shown are expressed as the means ± standard deviation of three independent experiments. (D) Expression of exogenous Myc-tagged Baf53a and Baf53b protein. Expression of either Myc-Baf53a or Myc-Baf53b was confirmed by Western blot analysis at 2 days after Tet treatment. αTubulin was used as a loading control. The result is representative of three independent experiments.
