Figure 1
The expression of CD44, CD24 and ALDH1 in different molecular subtypes of breast cancer. Four breast cancer cell lines were compared: MCF-7 (Luminal A subtype), SK-BR-3 (HER2 over expression subtype), MDA-MB-468 (basal-like epithelial subtype), and MDA-MB-231 (basal-like mesenchymal subtype). (A) Flow cytometry analysis of the expression of CD44 and CD24 in different breast cancer cell lines. Cells were double stained with anti-CD44-PE and anti-CD24-FITC. The threshold lines were set according to the isotype control. The accuracy of the double immunostaining was confirmed by comparison with single immunostaining for CD44 and CD24 respectively. (B) Immunofluorescence images showing the expression of CD44 and CD24 in different subtypes of breast cancer cell lines. Cells were labeled with anti-CD44-PE, anti-CD24–FITC and the nuclei stain DAPI. Scale bar: 20 μm. (C) The ratio of CD44/CD24 in different subtypes of breast cancer cell lines. Ratios were calculated from the percentage of CD44 and CD24 positive subpopulations in the flow cytometry analysis. Data represent means ± SD (n = 3), **P < 0.01, ***P < 0.001, ****P < 0.0001. (D) Immunofluorescence images showing the expression of ALDH1 in different breast cancer cell lines. Cells were labeled with anti-ALDH1 (magenta) and DAPI (blue), scale bar: 20 μm. (E) The average fluorescent intensities of ALDH1 in different breast cancer cell lines, data were calculated from three parallel immunofluorescence images. Data represent means ± SD (n = 3), ***P < 0.001, ****P < 0.0001.
