Figure 5

ALDH1 expression positively correlates with the expression of the tumor metastatic marker CXCR4. (A) Immunofluorescence images showing the expression of CXCR4 in different breast cancer cell lines. Cells were labeled with anti-CXCR4 (green) and DAPI (blue), scale bar: 20 μm. (B) The expression level of CXCR4 in in different breast cancer cell lines. The average fluorescence intensities were calculated from three parallel immunofluorescence images. Data represent means ± SD (n = 3), **P < 0.01, ***P < 0.001. (C) Western blot analysis of the expression of ALDH1, CXCR4 in different breast cancer cell lines. β-actin was used as control. The bolts were cropped from their original images and the full-length blots were presented in Supplementary Figure S5.