Figure 9
CTCs exhibit high CD44/CD24 ratio and ALDH1 expression. (A) Schematic illustration of the isolation and detection of CTCs from the blood using membrane filter. The isolation device contains an 8 μm pore filtering membrane and a 25 μm diameter filter holder. (B) Immunofluorescent images showing the expression of CK19, CD45, CD44, CD24 and ALDH1 in CTCs isolated from the immunodeficient mice injected with MDA-MB-231 cells. CTCs captured on the 8 μm polycarbonate membrane were stained with anti-CK19 (green), anti-CD45, anti-CD44 (red), anti-CD24 (green), anti-ALDH1 (magenta) and DAPI (blue), respectively. Scale bar: 20 μm. (C) Immunofluorescent images showing the expression of CD44, ALDH1 and CD45 in CTCs isolated from breast cancer patient. CTCs captured on the 8 μm polycarbonate membrane were stained with anti-CD44 (green), anti-ALDH1 (magenta), anti-CD45 (red), and DAPI (blue), respectively. Scale bar: 20 μm.
