Figure 5
From: IFN-γ and TNF-α synergism may provide a link between psoriasis and inflammatory atherogenesis
IFN-γ and TNF-α synergistically increase expression of endothelial cell activation markers in vitro and in vivo. Human aortic endothelial cells (HAoECs) stimulated with IFN-γ, TNF-α or a combination demonstrate increased (a) mRNA expression following 6 hours of stimulation and surface expression (shown as Mean Fluorescent Intensity (MFI)) of endothelial cell activation markers following (b) 4 and (c) 24 hours of stimulation, confirming a synergistic effect between IFN-γ and TNF-α (*p < 0.05, **p < 0.001, ***p < 0.0001, two-way ANOVA). (d,e) HAoEC barrier function meausred by ECIS demostrates a decrease in resistance of HAoECs following 6, 12 and 20 hours of IFN-γ and TNF-α treatment p < 0.01, WSANOVA). Data are shown as mean ± SD with 95% CI.
