Figure 3

Detection of ROS production. (A) Detection of CellRox Green fluorescence after ICG-NIR in vitro (x200). (B) Immunohistochemical analysis of 8-OHdG after ICG-NIR in tumor xenograft model (x400). 8-OHdG is a DNA marker of oxidative damage and positive staining of 8-OHdG indicates ROS production. (C) Detection of CellRox Green fluorescence and the ratio of fluorescence intensity of ICG-NIR with or without heat storage sheet in vitro (x200). HEAT: with heat storage sheet, COLD: without heat storage sheet.